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| Registros recuperados: 27 | |
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| Bolívar,Ana María; Rojas,Agustina; Rosales,Datty; Torres,Yzoleth; García-Lugo,Pablo. |
| La siguiente investigación presenta los resultados de las evaluaciones diagnósticas, realizadas por PCR, para agentes hemotrópicos, en ganado bufalino y bovino (n=77), coexistentes en una misma explotación agropecuaria. Se obtuvo una positividad de 74,03% de infección hemoparásita para el total de la población estudiada, con predominio de Anaplasma marginale sobre Trypanosoma theileri, Trypanosoma vivax y Babesia bigemina, respectivamente. Adicionalmente, algunos de los ADN problema fueron ensayados por DGGE, revelando la presencia de A. marginale y Mycoplasma wenyonii. Los resultados obtenidos corroboran la clínica presuntiva y el valor del método molecular. |
| Tipo: Journal article |
Palavras-chave: Ganado vacuno; Ganado bufalino; Hemotrópicos; PCR; DGGE. |
| Ano: 2014 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2014000100009 |
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| Carmo,F.L.; Santos,H.F.; Peixoto,R.S.; Rosado,A.S.; Araujo,F.V.. |
| The Atlantic Rainforest does not have a uniform physiognomy, its relief determines different environmental conditions that define the composition of its flora and fauna. Within this ecosystem, bromeliads that form tanks with their leaves hold water reservoirs throughout the year, maintaining complex food chains, based mainly on autotrophic and heterotrophic bacteria. Some works concluded that the water held by tank bromeliads concentrate the microbial diversity of their ecosystem. To investigate the bacterial diversity and the potential biotechnology of these ecosystems, tank bromeliads of the Neoregelia cruenta species from the Atlantic Rainforest in Brazil were used as models for this research. Bacteria isolated from these models were tested for... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Bromeliad; Atlantic Rainforest; Bacteria communities; DGGE. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100024 |
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| COUTINHO, H. L. da C.; ABOIM, M. C. R.; ROSADO, A. S.; BARBOSA, J. C.. |
| Qualidade do solo é um conceito, desenvolvido a partir da década de 1980 e aprimorado na década seguinte, que considera não apenas a função do solo em prover nutrientes e substrato físico para o crescimento de plantas, mas também sua capacidade de regular o clima e a hidrologia do ecossistema onde se insere, e funcionando desta forma harmoniosa para atender não só as necessidades das gerações presentes, mas também das gerações futuras da humanidade. Assim, qualidade do solo é um conceito intimamente ligado ao de sustentabilidade, de onde se conclui que não há sistema de uso da terra sustentável se a qualidade do solo se deteriora ao longo do tempo. O monitoramento dos impactos, positivos ou negativos, do uso da terra sobre a qualidade do solo demanda a... |
| Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) |
Palavras-chave: Qualidade do solo; Diversidade microbiana; DGGE; Mata Atlântica; Agricultura migratória. |
| Ano: 2004 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/964812 |
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| Fracetto,Giselle G. M.; Azevedo,Lucas C.B.; Fracetto,Felipe J. C.; Andreote,Fernando D.; Lambais,Marcio R.; Pfenning,Ludwig H.. |
| Considered as one of the most biodiverse biomes, the Amazon has a featured role in the discovery of new species of plants, animals and microorganisms, which may be important for the functionality of different ecosystems. However, studies on the impacts resulted from changes in the Amazon land use on microbial communities and their functions are still limited. In this context, the soil fungal diversity can act as an important indicator of environmental stress caused by land use of the Amazon. This study describes changes in soil fungal communities caused by different systems of land use (primary forest, secondary forest, agroforestry, agriculture and pasture). Communities were observed in each of the areas using denaturing gradient gel electrophoresis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DGGE; 18S rRNA; Fungal diversity; Soil biology. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000200001 |
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| Gatesoupe, Joel; Coves, Denis; Ortega, Aurelio; Papandroulakis, Nikos; Vadstein, Olav; De La Gandara, Fernando. |
| During the first 2 years of larval rearing trials with Atlantic bluefin tuna, survival was a challenging issue. As bacterial colonization of the gut has been shown to play a key role for other species, we studied the profiles of the microbiota associated with individual larvae at different stages in three distant hatcheries. The Bacterial Community Profile (BCP) was quantified based on PCR-DGGE analyses of partial amplicons from 16S rDNA. Considerable individual variability in BCP was observed before onset of feeding, and the BCP did not show regular fluctuation during ontogenesis. Microalgae were added to the rearing tanks in two of the three hatcheries, but it was not possible to distinguish the effect of location from the effect of algal addition on... |
| Tipo: Text |
Palavras-chave: Larval rearing; Bacterial diversity; DGGE; Thunnus thynnus. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00075/18648/16197.pdf |
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| He,Suxu; Zhou,Zhigang; Banerjee,Goutam; Huang,Lu; Ray,Arun Kumar; Ringø,Einar. |
| ABSTRACT An investigation was conducted to identify the allochthonous microbiota (entire intestine) and the autochthonous microbiota in proximal intestine (PI) and distal intestine (DI) of four species of Indian air-breathing fish (climbing perch; Anabas testudineus, murrel; Channa punctatus, walking catfish; Clarias batrachus and stinging catfish; Heteropneustes fossilis) by PCR based denaturing gradient gel electrophoresis (DGGE). High similarities of the allochthonous microbiota were observed between climbing perch and murrel, walking catfish and stinging catfish, indicating similar food behavior. The autochthonous microbiota of PI and DI from climbing perch and murrel revealed more similarity, than the result obtained from walking catfish and stinging... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allochthonous and autochthonous gut microbiota; Air-breathing fish; Bacterial diversity; DGGE. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100350 |
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| Knupp,Adriano Moreira; Martins,Claudia Miranda; Faria,Josias Corrêa de; Rumjanek,Norma Gouvêa; Xavier,Gustavo Ribeiro. |
| O objetivo deste trabalho foi avaliar o efeito do feijoeiro geneticamente modificado quanto à resistência ao Bean Golden Mosaic Vírus, BGMV (Olathe M1-4), sobre organismos não alvo. De um experimento implantado no campo, em delineamento inteiramente casualizado, com dois tratamentos (Olathe Pinto e evento elite Olathe M1-4), dois períodos amostrais (estádio V4 e R6) e dez repetições, obtiveram-se células bacterianas cultivadas e não cultivadas da rizosfera e do solo não rizosférico, para as quais se procedeu à extração de DNA total. A região V6-V8 do 16S rDNA foi amplificada para a comunidade bacteriana total, e também realizou-se amplificação com iniciadores específicos para o subgrupo alfa (α) do filo Proteobacteria a partir de células não cultivadas.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phaseolus vulgaris; Biossegurança; DGGE; Impacto ambiental; Organismos não alvo; Transgênico. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009001200019 |
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| Lamari, Faouzi; Castex, Mathieu; Larcher, Thibaut; Ledevin, Mireille; Mazurais, David; Bakhrouf, Amina; Gatesoupe, Joel. |
| Probiotics may have many effects on health and development of fish larvae. One of the most promising is related to spinal conformation, though the mode of action is not clearly understood. The present study attempted to investigate the effects of two strains of lactic acid bacteria on associated microbiota, histological development and gene expression. Sea bass larvae were fed since 5 dph (day post hatch) with either a standard control diet (Diet C), or the same diet supplemented with Pediococcus acidilactici MA18/5M (Diet P), or with an autochthonous strain of Lactobacillus casei (X2; Diet L). The two lactic acid bacteria were incorporated in the diets at the levels of 106 and 107 CFU (colony forming units) g-1 in two consecutive experiments,... |
| Tipo: Text |
Palavras-chave: Fish larvae; Probiotics; Microbiota; Histopathology; Gene expression; DGGE. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00108/21966/19673.pdf |
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| LAVIN,PARIS; GÓMEZ,PATRICIA; GONZÁLE,BERNARDO; ULLOA,OSVALDO. |
| In order to assess the appropriateness of the use of internal transcribed spacer (ITS) sequences for the study of population genetics of marine cyanobacteria, we amplified and cloned the 16S rRNA gene plus the 16S-23S ITS regions of six strains of Prochlorococcus and Synechococcus. We analyzed them by denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphisms (T-RFLP). When using the standard application of these techniques, we obtained more than one band or terminal restriction fragment (T-RF) per strain or cloned sequence. Reports in literature have suggested that these anomalies can result from the formation of secondary structures. Secondary structures of the ITS sequences of Prochlorococcus and Synechococcus... |
| Tipo: Journal article |
Palavras-chave: Cyanobacteria; DGGE; T-RFLP; ITS; Secondary structure. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2008000400006 |
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| Lazar, Cassandre Sara. |
| A large portion of the greenhouse gas methane on Earth is trapped in marine sediments. However, little methane actually reaches the hydrsphere and the atmosphere, because it is efficiently consumed by anaerobic methane-oxidizing Archaea (ANME). This methane is mainly biogenic in marine sédiments, and is produced by methanogenic Archaea. Methane ascends from deep sources to the seabed, in cold seep sites of continental margins. In order to study microbial community diversity in cold seeps, and to determine geochemical factors that control these microbial communities, we compared four geochemically and geologically distinct sites. In this work, molecular tools (PCR, RT-PCR, DGGE, cloning) as well as genetic markers (16S rRNA, mcrA, dsrB) were employed, to... |
| Tipo: Text |
Palavras-chave: Diversité moléculaire; Archaea; Méthanogène; ANME; McrA; DsrB; DGGE; Volcan de boue; Pockmark; Saumures; Fluides froids; Méthane; Mer de Norvège; Mer Méditerranée; Sédiments.; Molecular diversity; Archaea; Methanogen; ANME; McrA; DsrB; DGGE; Mud volcano; Pockmark; Brines; Cold seep; Methane; Norwegian Sea; Mediterranean Sea; Sediments.. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00031/14250/11534.pdf |
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| López-López,P.; Martínez-López,M.C.; Boldo-León,X.M.; Hernández-Díaz,Y.; González-Castro,T.B.; Tovilla-Zárate,C.A.; Luna-Arias,J.P.. |
| Amebiasis is one of the twenty major causes of disease in Mexico; however, the diagnosis is difficult due to limitations of conventional microscopy-based techniques. In this study, we analyzed stool samples using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to differentiate between Entamoeba histolytica (pathogenic) and E. dispar (non-pathogenic). The target for the PCR amplification was a small region (228 bp) of the adh112 gene selected to increase the sensitivity of the test. The study involved 62 stool samples that were collected from individuals with complaints of gastrointestinal discomfort. Of the 62 samples, 10 (16.1%) were positive for E. histolytica while 52 (83.9%) were negative. No sample was positive for E.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amebiasis; Neglected diseases; Diagnostic; PCR; DGGE; Adh112 gene. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400607 |
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| Nakatani,André Shigueyoshi; Siqueira,José Oswaldo; Soares,Cláudio Roberto Fonsêca Sousa; Lambais,Marcio Rodrigues. |
| As raízes das plantas podem estimular a microbiota do solo, a qual pode contribuir para o aumento da eficiência do processo de remediação. Assim, avaliar a magnitude dos efeitos das raízes sobre a microbiota do solo é de grande interesse e de relevância prática e ecológica. Neste trabalho, avaliaram-se a densidade microbiana, a atividade enzimática, a estrutura da comunidade bacteriana e a presença de fungos micorrízicos arbusculares (FMAs) na rizosfera de plantas de ocorrência espontânea em solo de sistema de "landfarming" de resíduos petroquímicos. Avaliaram-se também solos rizosféricos de cinco plantas e solo-controle sem planta por meio de contagens de microrganismos em placas, eletroforese em gel com gradiente desnaturante (DGGE) de fragmentos do gene... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rizosfera; Petróleo; Microrganismos degradadores de antraceno; Micorriza arbuscular; DGGE; RRNA 16S. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832008000400014 |
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| Reali,Catiusca; Pilz Júnior,Harry Luiz; Meile,Jean Christophe; Galindo,Sabine Shorr; Fiuza,Lidia Mariana. |
| ABSTRACT The microbial composition of different types,in ecosystems (including agro-ecosystems), has been investigated in a rapidly growing number of studies in the past few years. The importance of microorganisms, regarding the maintenance and stability of nutrients in agroecosystems, is a key to maintain the sustainability of a crop. Molecular tools to study microbial communities are possible through many methods such as RISA, DGGE, TGGE, clone libraries, T-RFLP, RAPD, SSCP and more recently NGS (Next-Generation Sequencing). DGGE is widely employed to characterize the diversity and the community dynamics of microorganisms in the environment, making possible to find out specific groups through functional genes, allowing access to data that cannot be... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agroecosystems; Functional genes; Bacteria; DGGE; Metagenomics; Microbial ecology. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100201 |
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| Registros recuperados: 27 | |
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